Serum Albumin by Dye-Binding: Bromocresol Green or Bromocresol Purple? The Case for Conservatism

Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.

BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...

Determination of caprine serum albumin in milk using bromocresol green dye

The objective of this study was to develop and validate a colorimetric assay to measure caprine serum albumin (CSA) concentrations in milk. The principle of the say was based on the binding of albumin to bromocresol green (BCG) dye at pH 4.0. The absorption of the dye-albumin complex was determined spectrophotometrically at 640 nm. A linear relationship existed between CSA concentrations (0.01 ...

Bromocresol purple, a non-specific colour reagent for the determination of serum albumin.

The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...

The introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure.

The bromocresol purple methods for the determination of serum albumin on the Technicon SMAC and the DuPont ACA systems were evaluated and compared with the corresponding bromocresol green methods, a radial immunodiffusion (RID) and an electrophoresis technique. The linearity and precision of the dye binding methods were comparable. The bromocresol purple methods were more influenced by lipaemia...

Plasma albumin determination on the Cobas Bio centrifugal analyser using bromocresol green